Biology Biotechnology Principles and Processes All Topic Question For Revision Test Single Correct MCQ
Published on: August 13, 2026

PCR proceeds in three distinct steps governed by temperature they are in order of :-

A
Denaturation, Annealing, Synthesis
B
Synthesis, Annealing, Denaturation
C
Annealing, Synthesis, Denaturation
D
Denaturation, Synthesis, Annealing

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Text Solution

Verified by Experts
The correct answer is:
A

The three steps of PCR (Polymerase Chain Reaction) are:

Denaturation (~94–95°C) → DNA strands separate.

Annealing (~50–65°C) → Primers bind to the template DNA.

Extension/Synthesis (~72°C) → DNA polymerase synthesizes new DNA strands.

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